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Such changes are often induced by the binding of a substrate molecule to an enzyme's active siteor the physical region of the protein that participates in chemical catalysis. The two different Sec-tRNA Ser Sec isoforms have different roles in the synthesis of the repertoire of human selenoproteins.

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Key experimental techniques in proteomics include 2D electrophoresis[49] which allows the writing profiles essay of a large number of proteins, mass spectrometry[50] which allows rapid high-throughput identification of proteins and sequencing of peptides most often after in-gel digestionprotein microarrayswhich allow the detection of the relative levels of a large number of proteins present in a cell, and two-hybrid screeningwhich allows the systematic exploration of protein—protein interactions. As a result, when the lysate is passed over a chromatography column containing nickelthe histidine residues ligate the nickel and attach to the column while the untagged components of the lysate pass unimpeded.

Coagulation the process involved in blood clot formation.

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Working with Lafayette Mendel and applying Liebig's law of the minimum in control laboratory ratsthe nutritionally essential amino acids were established. Proper folding occurs prior to the transfer of the protein to the Golgi apparatus.

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In animals, amino acids are obtained through the consumption of foods containing protein.